This experiment investigated caffeine's effect on asparagus root development and the absorption of minerals. Previous studies have tested different concentrations of caffeine in a nutrient medium; thus, obtaining data on caffeine’s effect under optimal nutrient conditions becomes necessary. The influence of caffeine on plant yield and seed germination rates changes depending on different concentrations. To solidify the effects of different concentrations, this experiment has been conducted using concentrations of decaffeinated and caffeinated green tea. Given the prolonged germination period of asparagus seedlings, a pre-planting soaking procedure in water was done for 24 hours to enhance germination. After soaking the seedlings, they were placed in rockwool to germinate. Data was collected weekly on the root growth, and the fresh weight was collected after the experiment. By week 4, the root growth of 1% decaffeinated green tea exceeded threefold of what was observed in the control group. After week 4, all groups excluding 1% decaffeinated exhibited less root development than the control group. Concentrations of 2% or concentrations that contain caffeine restrict root growth and fresh weight. The fresh weight of seedlings that received caffeinated treatments is half that of seedlings treated with 1% decaffeinated solution. Notably, the sprouting ratio was most influenced by green tea concentrations rather than caffeine content, with 2% decaffeinated and caffeinated concentrations exhibiting the greatest sprouting ratio. With high root length and full sprouting ratio, asparagus seedlings treated with 1% decaffeinated green tea indicated the inefficiency of caffeine on the germination rate of asparagus seedlings.
Abstract This experiment investigated caffeine's effect on asparagus root development and the absorption of minerals. Previous studies have tested different concentrations of caffeine [...]
This experiment analyzed the effects of pomegranate juice on C. elegans under thermal stress. C. elegans, a nematode with similar genes to humans, is an effective model for studying human diseases. Thermal stress occurs when an organism is exposed to high temperatures, which results in the release of heat shock proteins. Heat shock proteins act as molecular chaperones that help restore protein homeostasis following heat stress. Despite their presence, thermal stress can still weaken antioxidant defense in organisms. However, pomegranate juice is rich in antioxidants and polyphenols, which can help strengthen antioxidant defense. In this study, pomegranate extract was dissolved in water and mixed with agar powder. Using this pomegranate mixed agar, E. coli was cultured for 24 hours. The results showed that the 5 mg/ml concentration increased survival rates of C. elegans. However, the 10 and 20 mg/ml concentrations lowered survival rates, which can be attributed to dose-dependent toxicity. Interestingly, the higher concentrations of pomegranate extract significantly increased C. elegans reproductive rates. This research expands on previous studies that examined the effect of pomegranate juice on C. elegans and distinctly focuses on its role under thermal stress. This distinction helps gain a deeper insight into pomegranate juice's effect on oxidative stress.
Abstract This experiment analyzed the effects of pomegranate juice on C. elegans under thermal stress. C. elegans, a nematode with similar genes to humans, is an effective model [...]
Collection of Articles on Biochemistry, Genetics and Molecular Biology (2025). 1
Abstract
This experiment analyzed the effects of pomegranate juice on C. elegans under thermal stress. C. elegans, a nematode with similar genes to humans, is an effective model for studying human diseases. Thermal stress occurs when an organism is exposed to high temperatures, which results in the release of heat shock proteins. Heat shock proteins act as molecular chaperones that help restore protein homeostasis following heat stress. Despite their presence, thermal stress can still weaken antioxidant defense in organisms. However, pomegranate juice is rich in antioxidants and polyphenols, which can help strengthen antioxidant defense. In this study, pomegranate extract was dissolved in water and mixed with agar powder. Using this pomegranate mixed agar, E. coli was cultured for 24 hours. The results showed that the 5 mg/ml concentration increased survival rates of C. elegans. However, the 10 and 20 mg/ml concentrations lowered survival rates, which can be attributed to dose-dependent toxicity. Interestingly, the higher concentrations of pomegranate extract significantly increased C. elegans reproductive rates. This research expands on previous studies that examined the effect of pomegranate juice on C. elegans and distinctly focuses on its role under thermal stress. This distinction helps gain a deeper insight into pomegranate juice's effect on oxidative stress.
Abstract This experiment analyzed the effects of pomegranate juice on C. elegans under thermal stress. C. elegans, a nematode with similar genes to humans, is an effective [...]
Armaanjit Singh's personal collection 
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The effect of Caffeine on the rhizogenesis of Asparagus seeds 
