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Purpose: The aim of this study was to investigate and compare the effects of high-fructose, high-fat diet supplemented with soy bean oil or coconut, on the testicular testosterone biosynthesis, antioxidant capability and cell apoptosis in male rats.
Materials and Methods: Wistar rats were 24 male, 6 weeks old and divided into 3 groups: control group, soybean oil group and coconut oil group. Soybean oil group and coconut oil group were fed with high fat (39 % of total calorie) high fructose (48 % of total calorie) diets. After 20 weeks, rats were given sacrificed.
Results: The soybean oil group had lowest serum testosterone level and saturated fatty acid ratio in testis fatty acid composition; the expression of Cytochrome P450 (CYP17A1) involved in testosterone biosynthesis and glutathione peroxidase activity was highest among groups. Also enhanced Tumor necrosis factor α (TNF α) and Nuclear factor kappa-light-chain-enhancer of activated B cells (NF κB). Moreover, western blot analysis clearly showed the significantly increased testicular cleavage caspase 3 and PARP (poly ADP ribose polymerase) expression in the soybean group.
Conclusion: These results suggest that high-fructose and high-soybean diet decreased reproductive function due to modify testicular fatty acid composition, increased the oxidative stress in the testis and germ cell apoptosis in male rats.
Published on 04/10/16
Licence: Other
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