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== Abstract ==
 
== Abstract ==
  
Two non-destructive techniques, confocal laser scanning microscopy (CLSM) and planar optode (VisiSens imaging), were combined to relate the fine-scale spatial structure of biofilm components to real-time images of oxygen decay in aquatic biofilms. Both techniques were applied to biofilms grown for seven days at contrasting light and temperature (<math>10/20°C</math>) conditions. The geo-statistical analyses of CLSM images indicated that biofilm structures consisted of small (~100 µm) and middle sized (<math>~101 \mu m</math>) irregular aggregates. Cyanobacteria and EPS (extracellular polymeric substances) showed larger aggregate sizes in dark grown biofilms while, for algae, aggregates were larger in light<math>-20°C</math> conditions. Light<math>-20°C</math> biofilms were most dense while <math>10°C</math> biofilms showed a sparser structure and lower respiration rates. There was a positive relationship between the number of pixels occupied and the oxygen decay rate. The combination of optodes and CLMS, taking advantage of geo-statistics, is a promising way to relate biofilm architecture and metabolism at the micrometric scale.
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Two non-destructive techniques, confocal laser scanning microscopy (CLSM) and planar optode (VisiSens imaging), were combined to relate the fine-scale spatial structure of biofilm components to real-time images of oxygen decay in aquatic biofilms. Both techniques were applied to biofilms grown for seven days at contrasting light and temperature (<math>10/20ºC</math>) conditions. The geo-statistical analyses of CLSM images indicated that biofilm structures consisted of small (~100 µm) and middle sized (<math>~101 \mu m</math>) irregular aggregates. Cyanobacteria and EPS (extracellular polymeric substances) showed larger aggregate sizes in dark grown biofilms while, for algae, aggregates were larger in light<math>-20ºC</math> conditions. Light<math>-20ºC</math> biofilms were most dense while <math>10ºC</math> biofilms showed a sparser structure and lower respiration rates. There was a positive relationship between the number of pixels occupied and the oxygen decay rate. The combination of optodes and CLMS, taking advantage of geo-statistics, is a promising way to relate biofilm architecture and metabolism at the micrometric scale.
  
 
==Full Document==
 
==Full Document==
  
 
<pdf>Media:Draft_Samper_789097209_4059_Biofouling+Rubol_et_al_2018.pdf</pdf>
 
<pdf>Media:Draft_Samper_789097209_4059_Biofouling+Rubol_et_al_2018.pdf</pdf>

Revision as of 14:31, 27 March 2020

Abstract

Two non-destructive techniques, confocal laser scanning microscopy (CLSM) and planar optode (VisiSens imaging), were combined to relate the fine-scale spatial structure of biofilm components to real-time images of oxygen decay in aquatic biofilms. Both techniques were applied to biofilms grown for seven days at contrasting light and temperature (Failed to parse (syntax error): 10/20ºC ) conditions. The geo-statistical analyses of CLSM images indicated that biofilm structures consisted of small (~100 µm) and middle sized () irregular aggregates. Cyanobacteria and EPS (extracellular polymeric substances) showed larger aggregate sizes in dark grown biofilms while, for algae, aggregates were larger in lightFailed to parse (syntax error): -20ºC

conditions. LightFailed to parse (syntax error): -20ºC
biofilms were most dense while Failed to parse (syntax error): 10ºC
biofilms showed a sparser structure and lower respiration rates. There was a positive relationship between the number of pixels occupied and the oxygen decay rate. The combination of optodes and CLMS, taking advantage of geo-statistics, is a promising way to relate biofilm architecture and metabolism at the micrometric scale.

Full Document

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Published on 01/01/2018

DOI: 10.1080/08927014.2017.1423474
Licence: CC BY-NC-SA license

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